PAX8 antibody (Internal Region)

Catalog No. ABIN190912

This Goat Polyclonal antibody specifically detects PAX8 in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for PAX8 antibody (Internal Region) (ABIN190912)

Target: PAX8 (Paired Box 8 (PAX8))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PAX8 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-PAX8 Antibody

Binding Specificity: Internal Region

Purpose: PAX8 (internal)

Sequence: AQPGSDKRKM DD

Specificity: This antibody is expected to recognise all reported isoforms (NP_003457.1, NP_039245.1, NP_039246.1, NP_039247.1, NP_054698.1)

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-AQPGSDKRKMDD, from the internal region of the protein sequence according to NP_003457.1, NP_039245.1, NP_039246.1, NP_039247.1, NP_054698.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: In transfected HEK293 transiently expressing PAX8A a band of approx. 55 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.5-1.5 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PAX8

Target: PAX8 (Paired Box 8 (PAX8))

Alternative Name: PAX8

Background: PAX8, paired box gene 8, HGNC:8622 , paired domain gene 8, paired box 8

Gene ID: 7849

NCBI Accession: NP_003457, NP_039245, NP_039246, NP_039247, NP_054698

Pathways: Thyroid Hormone Synthesis, Regulation of Hormone Metabolic Process, Stem Cell Maintenance, Tube Formation