COL7A1 antibody (Internal Region)

Catalog No. ABIN190915

This Goat Polyclonal antibody specifically detects COL7A1 in WB and ELISA. It exhibits reactivity toward Rat.

Quick Overview for COL7A1 antibody (Internal Region) (ABIN190915)

Target: COL7A1 (Collagen, Type VII, alpha 1 (COL7A1))

Reactivity: Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This COL7A1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-COL7A1 Antibody

Binding Specificity: Internal Region

Purpose: Collagen, type VII

Sequence: TVQYSDDPRT EF

Specificity: This product does not work on cryostat sections of Normal Human Skin.

Cross-Reactivity: Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: C-TVQYSDDPRTEF

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 250 kDa band observed in Rat Testis lysates (calculated MW of 159 kDa according to Rat NP_001100328.1). Recommended concentration: 0.1-1.5 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

A customer found this antibody unsuccessful on cryosections of Human Skin.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for COL7A1

Target: COL7A1 (Collagen, Type VII, alpha 1 (COL7A1))

Alternative Name: COL7A1

Background: Collagen, type VII, collagen, type VII, alpha 1 (epidermolysis bullosa, dystrophic, dominant and recessive), COL7A1, HGNC:2214, EBD1, EBDCT, EBR1, LC collagen, alpha 1 type VII collagen, collagen VII, alpha-1 polypeptide, long chain collagen, collagen, ty

Gene ID: 1294, 12836

NCBI Accession: NP_000085