OSBPL5 antibody (N-Term)

Catalog No. ABIN190918

This Goat Polyclonal antibody specifically detects OSBPL5 in WB, ELISA, IF, FACS and IHC. It exhibits reactivity toward Human and Rat.

Quick Overview for OSBPL5 antibody (N-Term) (ABIN190918)

Target: OSBPL5 (Oxysterol Binding Protein-Like 5 (OSBPL5))

Reactivity: Human, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This OSBPL5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-OSBPL5 Antibody

Binding Specificity: N-Term

Purpose: ORP5 (OBPH1)

Sequence: KEEAFLRRRF SLC

Specificity: This antibody is expected to recognise both reported isoforms. Variants (NP_663613.1, NP_001137535.1) encode the same isoform.

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence KEEAFLRRRFSLC, from the N Terminus of the protein sequence according to NP_065947.1, NP_663613.1, NP_001137535.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Kidney and Liver. Recommended concentration: 5 μg/mL.

Western Blot: Approx. 100 kDa band observed in lysates of cell line Jurkat and in Rat Spleen lysates (calculated MW of 98.4 kDa according to Human NP_065947.1 and 101.7 kDa according to Rat NP_001015024.2). Recommended concentration: 0.03=0.3 μg/mL. Primary

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa and U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration:

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for OSBPL5

Target: OSBPL5 (Oxysterol Binding Protein-Like 5 (OSBPL5))

Alternative Name: OSBPL5

Background: OSBPL5, ORP5, OBPH1, FLJ31948, KIAA1534, DKFZp586H1719, oxysterol binding protein-like 5, OSBP-related protein 5, oxysterol-binding protein homologue 1, oxysterol-binding protein-related protein 5, FLJ42929, oxysterol-binding protein-like protein 5

Gene ID: 114879, 79196

NCBI Accession: NP_065947, NP_663613, NP_001137535