Oxysterol Binding Protein-Like 5 (OSBPL5) (N-Term) antibody

Details for Product No. ABIN190918
Antigen
  • MGC109308
  • OSBPL5
  • OBPH1
  • ORP5
  • 1110006M06Rik
  • AI462538
  • Obph1
  • Osbp2
  • oxysterol binding protein-like 5
  • oxysterol binding protein like 5
  • Osbpl5
  • OSBPL5
  • osbpl5
Epitope
N-Term
6
1
1
1
Reactivity
Human
9
Host
Goat
6
2
1
Clonality
Polyclonal
Conjugate
Un-conjugated
1
1
1
1
1
1
Application
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
9
7
1
1
1
1
Options
Purpose ORP5 (OBPH1)
Immunogen Peptide with sequence KEEAFLRRRFSLC, from the N Terminus of the protein sequence according to NP_065947.1, NP_663613.1, NP_001137535.1.
Blocking Peptide Blocking peptide for this product available: ABIN370200
Sequence KEEAFLRRRF SLC
Isotype IgG
Specificity This antibody is expected to recognise both reported isoforms. Variants (NP_663613.1, NP_001137535.1) encode the same isoform.
Predicted Reactivity Human, Mouse, Rat, Dog
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Grade Verified
Antigen
Alternative Name OSBPL5 (OSBPL5 Antibody Abstract)
Background OSBPL5, ORP5, OBPH1, FLJ31948, KIAA1534, DKFZp586H1719, oxysterol binding protein-like 5, OSBP-related protein 5, oxysterol-binding protein homologue 1, oxysterol-binding protein-related protein 5, FLJ42929, oxysterol-binding protein-like protein 5
Gene ID 114879
NCBI Accession NP_065947, NP_663613, NP_001137535
Application Notes

DS WB Results: Approx 110 kDa band observed in Human Spleen lysates (calculated MW of 98.4 kDa according to NP_065947.1). Recommended concentration: 0.2-0.6 μg/mL. Primary incubation was 1 hour.

DS IHC Results: Paraffin embedded Human Kidney and Liver. Recommended concentration: 5 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
Supplier Images
Image no. 1 for anti-Oxysterol Binding Protein-Like 5 (OSBPL5) (N-Term) antibody (ABIN190918) ABIN190918 (5µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retri...
Image no. 2 for anti-Oxysterol Binding Protein-Like 5 (OSBPL5) (N-Term) antibody (ABIN190918) ABIN190918 (0.2µg/ml) staining of Human Spleen lysate (35µg protein in RIPA buffer). ...
Image no. 3 for anti-Oxysterol Binding Protein-Like 5 (OSBPL5) (N-Term) antibody (ABIN190918) ABIN190918 (2µg/ml) staining of human spleen lysate (35µg protein in RIPA buffer). Pr...
Image no. 4 for anti-Oxysterol Binding Protein-Like 5 (OSBPL5) (N-Term) antibody (ABIN190918) ABIN190918 (5µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrie...
Background publications Higashimoto, Soejima, Yatsuki, Joh, Uchiyama, Obata, Ono, Wang, Xin, Zhu, Masuko, Ishino, Hatada, Jinno, Iwasaka, Katsuki, Mukai: "Characterization and imprinting status of OBPH1/Obph1 gene: implications for an extended imprinting domain in human and mouse." in: Genomics, Vol. 80, Issue 6, pp. 575-84, 2002 (PubMed).

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