The Mouse Monoclonal anti-Transferrin antibody is suitable to detect Transferrin in samples from Human. It has been validated for WB, ELISA and ELISA (Detection).
Human transferrin complex between transferrin and soluble transferrin receptor. MAb 2A2 reacts with N-terminal domain of human transferrin with Kd=2.85 x 10-11 M. MAbs 2A2 and 1C10 do not cross-react with mouse, rabbit, bovine transferrin and with human lactoferrin. IgG1 for all MAbs
Detection of transferrin in serum by sandwich immunoassay. Detection of transferrin - transferrin receptor complex by sandwich ELISA. All MAbs are working in ELISA. Recommended pairs for transferrin detection by sandwich immunoassay (coating – conjugate): 8B9 – 11D3, 8B9 – 12A6 Recommended MAbs for transferrin-transferrin receptor complex detection by ELISA sandwich method using anti-transferrin receptor MAb 23D10 (cat.No # 4Tr26) as coating: 23D10 – 8B9, 23D10 – 11D3 At 10-6 M MAbs 2A2 and 1C10 inhibit proliferation of Con-A stimulated peripheral blood lymphocytes. MAb 2A2 is working in indirect immunofluorescence on fixed, paraffin embedded human brain tissue.
Restrictions
For Research Use only
Buffer
PBS, pH 7.4, 0.1 % sodium azide (NaN3) Material safety note: This product is sold as an antibody preparation for research use only. Standard Laboratory Practices should be followed when handling this material. Contains sodium azide (0.1 %) as preservative
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.