KAP1 antibody (AA 1-50)

Catalog No. ABIN1944042

This anti-KAP1 antibody is a Rabbit Polyclonal antibody detecting KAP1 in WB, IHC, IF, IP, ICC, IHC (p) and PLA. Suitable for Human, Mouse, Rabbit, Dog, Cow and Horse.

Quick Overview for KAP1 antibody (AA 1-50) (ABIN1944042)

Target: KAP1 (TRIM28) (Tripartite Motif Containing 28 (TRIM28))

Reactivity: Human, Mouse, Rabbit, Dog, Cow, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KAP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Proximity Ligation Assay (PLA)

Product Details anti-KAP1 Antibody

Binding Specificity: AA 1-50

Specificity: Region between residue 1 and 50 of human Tripartite Motif-Containing 28 using the numbering given in entry NP_005753.1 (GeneID 10155).

Purification: Immunoaffinity purified

Isotype: IgG

Application Details

Application Notes: Approved: ICC (1:500 - 1:2500), IF (1:500 - 1:2500), IHC, IHC-P (1:1000 - 1:5000), IP (1 - 4 μg/mg lysate), PLA (1:1000 - 1:10000), WB (1:1000 - 1:20000)

Usage: Immunohistochemistry: Antigen retrieval with citrate buffer  pH 6.0 is recommended for FFPE tissue sections. Human controls: Breast Carcinoma, Colon Carcinoma, HeLa Cells, Non-Small Cell Lung Cancer, Ovarian Carcinoma, Prostate Carcinoma, Skin Basal Cell Carcinoma.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-citrate/phosphate buffer,  pH 7-8, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C for up to 1 year.

Expiry Date: 12 months

Target Details for KAP1

Target: KAP1 (TRIM28) (Tripartite Motif Containing 28 (TRIM28))

Alternative Name: TRIM28 / KAP-1

Background: Name/Gene ID: TRIM28
Subfamily: TIF1
Family: Tripartite Motif

Synonyms: TRIM28, E3 SUMO-protein ligase TRIM28, KRIP-1, Nuclear corepressor KAP-1, KAP-1, KAP1, KRAB-interacting protein 1, TIF1B, RNF96, TIF1-beta, TIF1beta, Tripartite motif-containing 28, KRAB-associated protein 1, RING finger protein 96, TF1B, Tif1beta zinc finger protein, Tripartite motif containing 28

Gene ID: 10155

Pathways: Hedgehog Signaling, Positive Regulation of Response to DNA Damage Stimulus