SP1 antibody (AA 1-250)

Catalog No. ABIN1944791

There are 2+ publications for this product available. The Mouse Monoclonal anti-SP1 antibody is suitable to detect SP1 in samples from Human. It has been validated for WB, IF and FACS.

Quick Overview for SP1 antibody (AA 1-250) (ABIN1944791)

Target: SP1 (Sp1 Transcription Factor (SP1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: 1326CT463-109-176

Product Details anti-SP1 Antibody

Binding Specificity: AA 1-250

Purification: This antibody is purified through a protein G column, followed by dialysis against PBS.

Immunogen: This SP1 antibody is generated from a mouse immunized with a recombination protein from the human SP1.

Isotype: IgG1 kappa

Application Details

Application Notes: IF: 1:25. WB: 1:1000. FC: 1:25

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified monoclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

References for anti-SP1 antibody (ABIN1944791)

Takahara, Kanazu, Yanagisawa, Akanuma: "Heterogeneous Sp1 mRNAs in human HepG2 cells include a product of homotypic trans-splicing." in: The Journal of biological chemistry, Vol. 275, Issue 48, pp. 38067-72, (2000) (PubMed).

Kadonaga, Carner, Masiarz, Tjian: "Isolation of cDNA encoding transcription factor Sp1 and functional analysis of the DNA binding domain." in: Cell, Vol. 51, Issue 6, pp. 1079-90, (1988) (PubMed).

Target Details for SP1

Target: SP1 (Sp1 Transcription Factor (SP1))

Alternative Name: SP1

Background: Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR- alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter. Plays an essential role in the regulation of FE65 gene expression. In complex with ATF7IP, maintains telomerase activity in cancer cells by inducing TERT and TERC gene expression.

Molecular Weight: 80693

Gene ID: 6667

UniProt: P08047

Pathways: Regulation of Lipid Metabolism by PPARalpha, Myometrial Relaxation and Contraction