FAS antibody

Catalog No. ABIN1944804

This anti-FAS antibody is a Rabbit Polyclonal antibody detecting FAS in WB and IHC. Suitable for Human. This Primary Antibody has been cited in 4+ publications.

Quick Overview for FAS antibody (ABIN1944804)

Target: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FAS antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-FAS Antibody

Isotype: IgG1

Application Details

Application Notes: WB: 1:1000. IHC: 1:50-1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Storage: 4 °C,-20 °C

References for anti-FAS antibody (ABIN1944804)

Cascino, Papoff, De Maria, Testi, Ruberti: "Fas/Apo-1 (CD95) receptor lacking the intracytoplasmic signaling domain protects tumor cells from Fas-mediated apoptosis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 156, Issue 1, pp. 13-7, (1996) (PubMed).

Liu, Cheng, Mountz: "Differential expression of human Fas mRNA species upon peripheral blood mononuclear cell activation." in: The Biochemical journal, Vol. 310 ( Pt 3), pp. 957-63, (1995) (PubMed).

Cascino, Fiucci, Papoff, Ruberti: "Three functional soluble forms of the human apoptosis-inducing Fas molecule are produced by alternative splicing." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 6, pp. 2706-13, (1995) (PubMed).

Oehm, Behrmann, Falk, Pawlita, Maier, Klas, Li-Weber, Richards, Dhein, Trauth: "Purification and molecular cloning of the APO-1 cell surface antigen, a member of the tumor necrosis factor/nerve growth factor receptor superfamily. Sequence identity with the Fas antigen." in: The Journal of biological chemistry, Vol. 267, Issue 15, pp. 10709-15, (1992) (PubMed).

Target Details for FAS

Target: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Alternative Name: FAS

Background: Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death- inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS- mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro).

Molecular Weight: 37732 Da

Gene ID: 355

UniProt: P25445

Pathways: p53 Signaling, Apoptosis, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity