Phenylalanine Hydroxylase antibody (AA 127-161)

Catalog No. ABIN1944892

This Mouse Monoclonal antibody specifically detects Phenylalanine Hydroxylase in WB and IHC (p). It exhibits reactivity toward Human, Mouse and Rat. It has been mentioned in 4+ publications

Quick Overview for Phenylalanine Hydroxylase antibody (AA 127-161) (ABIN1944892)

Target: Phenylalanine Hydroxylase

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Phenylalanine Hydroxylase antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 717CT21-1-1

Product Details anti-Phenylalanine Hydroxylase Antibody

Binding Specificity: AA 127-161

Predicted Reactivity: B

Purification: This antibody is purified through a protein G column, followed by dialysis against PBS.

Immunogen: This PAH antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 127-161 amino acids from the Central region of human PAH.

Isotype: IgG1 kappa

Application Details

Application Notes: WB: 1:1000. IHC-P: 1:25. IHC-P: 1:25. IHC-P: 1:25

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified monoclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

References for anti-Phenylalanine Hydroxylase antibody (ABIN1944892)

Siltberg-Liberles, Steen, Svebak, Martinez: "The phylogeny of the aromatic amino acid hydroxylases revisited by characterizing phenylalanine hydroxylase from Dictyostelium discoideum." in: Gene, Vol. 427, Issue 1-2, pp. 86-92, (2008) (PubMed).

Miranda, Teigen, Thórólfsson, Svebak, Knappskog, Flatmark, Martínez: "Phosphorylation and mutations of Ser(16) in human phenylalanine hydroxylase. Kinetic and structural effects." in: The Journal of biological chemistry, Vol. 277, Issue 43, pp. 40937-43, (2002) (PubMed).

Cotton, McAdam, Jennings, Morgan: "A monoclonal antibody to aromatic amino acid hydroxylases. Identification of the epitope." in: The Biochemical journal, Vol. 255, Issue 1, pp. 193-6, (1989) (PubMed).

Kwok, Ledley, DiLella, Robson, Woo: "Nucleotide sequence of a full-length complementary DNA clone and amino acid sequence of human phenylalanine hydroxylase." in: Biochemistry, Vol. 24, Issue 3, pp. 556-61, (1985) (PubMed).

Target Details for Phenylalanine Hydroxylase

Target: Phenylalanine Hydroxylase

Alternative Name: PAH

Target Type: Chemical

Molecular Weight: 51862

Gene ID: 5053

UniProt: P00439