Vitronectin antibody (AA 1-310)

Catalog No. ABIN1948187

The Mouse Monoclonal anti-Vitronectin antibody is suitable to detect Vitronectin in samples from Human, Cow, Dog, Rabbit and Cat. It has been validated for WB, ELISA, IHC and IHC (p).

Quick Overview for Vitronectin antibody (AA 1-310) (ABIN1948187)

Target: Vitronectin (VTN)

Reactivity: Human, Cow, Dog, Rabbit, Cat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Vitronectin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 6F212

Product Details anti-Vitronectin Antibody

Binding Specificity: AA 1-310

Specificity: Highly specific for vitronectin. There is no evidence for cross-reactivity with other connective tissue proteins (fibronectin, elastin, collagen, laminin). Cross-reacts with vitronectin from cat, dog, rabbit and to a lesser extent with cow. Epitope is located within a cyanogen bromide cleavage fragment comprising aa 1-310. Binds nearly as well to native vitronectin as to denatured. It interferes with vitronectin-inhibition of the lysis of terminal complement complexes (TCC) and it inhibits vitronectin binding of PAI-1.

Purification: Protein A/G purified

Immunogen: Human vitronectin purified from plasma by heparin-affinity chromatography.

Type of Immunogen: Purified protein

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA (1:40000), IHC, IHC-P (10 μg/mL), WB (1:50)

Usage: Suitable for use in ELISA, Western Blot. ELISA: 1:40,000. Western Blot: 1:50.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Vitronectin

Target: Vitronectin (VTN)

Alternative Name: VTN / Vitronectin

Background: Name/Gene ID: VTN
Family: Matrix Protein

Synonyms: VTN, Epibolin, Serum-spreading factor, VNT, S-protein, Somatomedin B, V75, VN, Complement S-protein, Serum spreading factor, Vitronectin

Gene ID: 7448

Pathways: Autophagy, Smooth Muscle Cell Migration