RPA2 antibody (pSer4, Ser8)

Catalog No. ABIN1959438

This anti-RPA2 antibody is a Rabbit Polyclonal antibody detecting RPA2 in WB, IHC, IF, IHC (p), IP and PLA. Suitable for Human and Mouse.

Quick Overview for RPA2 antibody (pSer4, Ser8) (ABIN1959438)

Target: RPA2 (Replication Protein A2, 32kDa (RPA2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RPA2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Proximity Ligation Assay (PLA)

Product Details anti-RPA2 Antibody

Binding Specificity: pSer4, Ser8

Specificity: Human RPA2 / RFA2 / RPA34 (phospho-Ser4/Ser8)

Purification: Immunoaffinity purified

Immunogen: Dually phosphorylated synthetic peptide, which represented a portion of human replication protein A2, 32 kD surrounding phosphorylated serines that corresponded to positions 4 and 8 using the numbering given in entry NP_002937.

Type of Immunogen: Synthetic phospho-peptide

Isotype: IgG

Application Details

Application Notes: Approved: IF (1:250 - 1:1000), IHC, IHC-P (1:500 - 1:2000), IP (4 - 8 μg/mg lysate), PLA (1:1000 - 1:20000), WB (1:1000 - 1:10000)

Usage: Immunohistochemistry: Antigen retrieval with Tris-EDTA  pH 9.0 is recommended for FFPE tissue sections. Human controls: Breast Carcinoma, Lung Adenocarcinoma, Ovarian Carcinoma IHC MOUSE CONTROLS Colon Carcinoma CT26, Renal Cell Carcinoma.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-citrate/phosphate buffer,  pH 7-8, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C for up to 1 year.

Expiry Date: 12 months

Target Details for RPA2

Target: RPA2 (Replication Protein A2, 32kDa (RPA2))

Alternative Name: RPA2 / RFA2 / RPA34

Background: Name/Gene ID: RPA2

Synonyms: RPA2, RF-A protein 2, RP-A p34, RPA34, Replication protein A2, 32  kDa, RP-A p32, REPA2, Replication factor A protein 2, Replication protein A2 (32kD), RPA32

Gene ID: 6118

NCBI Accession: NP_002937

Pathways: Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA