POLR2A/RPB1 antibody (pSer2)

Catalog No. ABIN1959521

The Rabbit Polyclonal anti-POLR2A/RPB1 antibody has been validated for WB, IHC, IF, IHC (p) and PLA. It is suitable to detect POLR2A/RPB1 in samples from Human, Mouse, Rat, Zebrafish (Danio rerio), Cow, Dog and Xenopus laevis.

Quick Overview for POLR2A/RPB1 antibody (pSer2) (ABIN1959521)

Target: POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Cow, Dog, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLR2A/RPB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Proximity Ligation Assay (PLA)

Product Details anti-POLR2A/RPB1 Antibody

Binding Specificity: pSer2

Specificity: Human POLR2A / RNA polymerase II (phospho-Ser2)

Purification: Immunoaffinity purified

Immunogen: Phosphorylated synthetic peptide, which represented a portion of human RNA Polymerase II (GeneID 5430) around serine 2 of the C-terminal repeat YSPTSPS.

Type of Immunogen: Synthetic phospho-peptide

Isotype: IgG

Application Details

Application Notes: Approved: IF (1:100 - 1:500), IHC, IHC-P (1:200 - 1:1000), PLA (1:200 - 1:4000), WB (1:2500 - 1:20000)

Usage: Immunohistochemistry: Antigen retrieval with citrate buffer  pH 6.0 is recommended for FFPE tissue sections. Human controls: Breast Carcinoma, Colon Carcinoma, Lung Adenocarcinoma, Ovarian Carcinoma, Prostate Carcinoma, Stomach Adenocarcinoma.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline, 0.1 % BSA, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C for up to 1 year.

Expiry Date: 12 months

Target Details for POLR2A/RPB1

Target: POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))

Alternative Name: POLR2A / RNA polymerase II

Background: Name/Gene ID: POLR2A

Synonyms: POLR2A, HRPB220, HsRPB1, POLR2, RpIILS, RPOL2, RNA Polymerase II, RPO2, POLRA, RNA polymerase II subunit B1, RPB1, RPBh1

Gene ID: 5430

Pathways: Regulatory RNA Pathways