HLA-ABC antibody

Catalog No. ABIN1959992

The Mouse Monoclonal anti-HLA-ABC antibody has been validated for FACS, IP, IHC (fro), IHC and ELISA. It is suitable to detect HLA-ABC in samples from Human and Cow.

Quick Overview for HLA-ABC antibody (ABIN1959992)

Target: HLA-ABC

Reactivity: Human, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This HLA-ABC antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (IHC), ELISA

Clone: MEM-123

Product Details anti-HLA-ABC Antibody

Brand: IHC-plus™

Specificity: Reacts with all human classical MHC Class I molecules (major histocompatibility complex) in native cell-surface forms as well as with human HLA-G cDNA transfected cells. MHC Class I molecules (MHC Class Ia) are expressed on the surface of all human cell types. The antibody MEM-123 completely blocks binding of classical W6/32 to surface-expressed HLA-G, but does not cross-blocks the antibody MEM-G/9

Purification: Protein A purified

Immunogen: COS-7 African green monkey kidney cells.

Isotype: IgG3

Application Details

Application Notes: Approved: ELISA, Flo (4 μg/mL), IHC, IHC-Fr (10 μg/mL), IP

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris buffered saline (TBS), 15 mM sodium azide, approx,  pH 8.0.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for HLA-ABC

Target: HLA-ABC

Alternative Name: HLA-A/B/C

Pathways: TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints