BCR antibody (pTyr177)

Catalog No. ABIN196938

Product Details anti-BCR Antibody

Target: BCR (Breakpoint Cluster Region (BCR))

Binding Specificity: pTyr177

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BCR antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of Bcr only when phosphorylated at Tyrosine 177.

Purification: Affinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from Human Bcr around the phosphorylation site of Tyrosine 177 (P-F-Yp-V-N).

Application Details

Application Notes: Suitable for use in Western blot (1/500-1/1000) and Immunohistochemistry onParaffin-Embedded Sections (1/50-1/100).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C.

Target Details for BCR

Target: BCR (Breakpoint Cluster Region (BCR))

Alternative Name: BCR (BCR Products)

Synonyms: si:dkey-83d9.7 antibody, zK83D9.7 antibody, BCR antibody, ALL antibody, BCR1 antibody, CML antibody, D22S11 antibody, D22S662 antibody, PHL antibody, 5133400C09Rik antibody, AI561783 antibody, AI853148 antibody, mKIAA3017 antibody, breakpoint cluster region antibody, BCR, RhoGEF and GTPase activating protein antibody, bcr antibody, BCR antibody, Bcr antibody

Background: The breakpoint cluster region protein (Bcr) is best know to be involved in genomic translocation with fusion partner Abl (Cbr-Abl) causing chronic myelogenous leukemia (CML). This 160 kDa protein contains a serine/threonine kinase domain, an SH2 binding domain, a GTP/GDP exchange domain and a C-term domain which functions as a GTPase activating protein for p21rac and CDC42. Additionally, Bcr is involved in signal transduction and can down regulate Ras mediated cell signaling.Synonyms: BCR1, Breakpoint cluster region protein, D22S11, NY-REN-26, Renal carcinoma antigen NY-REN-26

Gene ID: 613

NCBI Accession: NP_004318

UniProt: P11274

Pathways: Regulation of Leukocyte Mediated Immunity, Platelet-derived growth Factor Receptor Signaling