HDAC5 antibody (AA 496-500)

Catalog No. ABIN197340

The Rabbit Polyclonal anti-HDAC5 antibody (ABIN197340) specifically detects HDAC5 in WB and IHC (p). The antibody is reactive with Human samples.

Quick Overview for HDAC5 antibody (AA 496-500) (ABIN197340)

Target: HDAC5 (Histone Deacetylase 5 (HDAC5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HDAC5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-HDAC5 Antibody

Binding Specificity: AA 496-500

Specificity: HDAC5 antibody AP02669PU detects endogenous levels of total HDAC5 protein.

Purification: Immunoaffinity Chromatography using epitope-specific immunogen.

Immunogen: Peptide Sequenece around amino acids 496~500 (T-Q-S-S-P) derived from Human HDAC5.

Application Details

Application Notes: Suitable for use in Western blot (1: 500-1: 1000) and Immunohistochemistry (1: 50-1: 100).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C.

Target Details for HDAC5

Target: HDAC5 (Histone Deacetylase 5 (HDAC5))

Alternative Name: HDAC5

Background: HDAC5 is a member of the class II mammalian histone deacetylase family, which is structurally related to yeast HDA1. Human HDAC5 is composed of 1122 amino acid residues. The deacetylase domain of HDAC5 is located at the C-terminal half of the molecule. The N-terminal non-deacetylase domain does not show any significant homology with any published sequence.Both domains are required for HDAC5-mediated repression of gene transcription. HDAC5 interacts with a growing number of transcriptional factors including MEF2A as well as other HDAC proteins. The interacting complexes bind to specific regions of chromatin and regulate gene transcription in these regions.Synonyms: Antigen NY-CO-9, HD5, Histone deacetylase 5, KIAA0600

Gene ID: 10014

NCBI Accession: NP_001015053

UniProt: Q9UQL6

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Monocarboxylic Acid Catabolic Process