FOXO4 antibody (Ser197)

Catalog No. ABIN197368

This Rabbit Polyclonal antibody specifically detects FOXO4 in WB and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for FOXO4 antibody (Ser197) (ABIN197368)

Target: FOXO4 (Forkhead Box O4 (FOXO4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FOXO4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-FOXO4 Antibody

Binding Specificity: Ser197

Specificity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. AFX antibody AP02683PU detects endogenous levels of total AFX protein.

Purification: Immunoaffinity chromatography.

Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human AFX around the phosphorylation site of serine 197 (A-A-Sp-M-D).

Application Details

Application Notes: Suitable for use in Western blot (1: 500-1: 1000) and Immunohistochemistry (1: 50-1: 100).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C.

Target Details for FOXO4

Target: FOXO4 (Forkhead Box O4 (FOXO4))

Alternative Name: FOXO4 / AFX1 / MLLT7

Background: FOXO4 is a forkhead transcription factor involved in the regulation of the insulin signaling pathway. It binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. FOXO4 down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. It is also involved in negative regulation of the cell cycle.Synonyms: Fork head domain transcription factor AFX1, Forkhead box protein O4

Gene ID: 4303

NCBI Accession: NP_001164402

UniProt: P98177

Pathways: Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Muscle Cell Differentiation