ZAP70 antibody (Tyr319)

Catalog No. ABIN197384

The Rabbit Polyclonal anti-ZAP70 antibody has been validated for WB and IHC (p). It is suitable to detect ZAP70 in samples from Human and Mouse.

Quick Overview for ZAP70 antibody (Tyr319) (ABIN197384)

Target: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZAP70 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ZAP70 Antibody

Binding Specificity: Tyr319

Specificity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. Zap-70 antibody AP02691PU detects endogenous levels of total Zap-70 protein.

Purification: Immunoaffinity chromatography.

Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human Zap-70 around the phosphorylation site of tyrosine 319 (S-P-Yp-S-D).

Application Details

Application Notes: Suitable for use in Western blot (1: 500-1: 1000) and Immunohistochemistry (1: 50-1: 100).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C.

Target Details for ZAP70

Target: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Alternative Name: ZAP70

Background: Synonyms: 70 kDa zeta-associated protein, SRK, Syk-related tyrosine kinase, ZAP 70, ZAP-70

Gene ID: 7535

NCBI Accession: NP_001070

UniProt: P43403

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway