PRKAA1 antibody (Thr174)

Catalog No. ABIN197410

This Rabbit Polyclonal antibody specifically detects PRKAA1 in WB, IHC (p) and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for PRKAA1 antibody (Thr174) (ABIN197410)

Target: PRKAA1 (Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRKAA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Product Details anti-PRKAA1 Antibody

Binding Specificity: Thr174

Specificity: This antibody AP02704PU detects endogenous levels of total AMPK alpha-1/AMPK alpha-2 protein.

Purification: Immunoaffinity Chromatography using epitope-specific immunogen.

Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human AMPK1 alpha-1 around the phosphorylation site of threonine 174 (L-R-Tp-S-C).

Application Details

Application Notes: Western Blot: 1/500-1/1000. Immunofluorescence: 1/100-1/200. Immunohistochemistry on Paraffin Sections 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C.

Target Details for PRKAA1

Target: PRKAA1 (Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1))

Alternative Name: PRKAA1

Background: Synonyms: 5'-AMP-activated protein kinase catalytic subunit alpha-1/2, AMPK alpha-1, AMPK alpha-2, AMPK1, AMPK2, PRKAA1, PRKAA2

Gene ID: 5562

NCBI Accession: NP_006242

UniProt: Q13131

Pathways: AMPK Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, Warburg Effect