GAP43 antibody (Ser41)

Catalog No. ABIN197546

The Rabbit Polyclonal anti-GAP43 antibody has been validated for WB, IF and IHC (p). It is suitable to detect GAP43 in samples from Human, Rat and Mouse.

Quick Overview for GAP43 antibody (Ser41) (ABIN197546)

Target: GAP43 (Growth Associated Protein 43 (GAP43))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GAP43 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-GAP43 Antibody

Binding Specificity: Ser41

Specificity: This antibody detects endogenous levels of total GAP43 protein.

Purification: Immunoaffinity Chromatography using epitope-specific immunogen.

Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human GAP43 around the phosphorylation site of serine 41 (Q-A-Sp-F-R).

Application Details

Application Notes: Western blot: 1/500approx. 1/1000. Immunofluorescence: 1/100approx. 1/200. Immunohistochemistry on Paraffin-Embedded Sections: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C.

Target Details for GAP43

Target: GAP43 (Growth Associated Protein 43 (GAP43))

Alternative Name: GAP43

Background: GAP43 is expressed by developing and regenerating neurons, and to a lesser extent, reactive glial cells. It is used widely to specifically label injured neurons and to score neuronal regeneration. GAP43 is also a neuronal growth cone protein thought to be involved in pathfinding.Synonyms: Axonal membrane protein GAP-43, Growth-associated protein 43, Neural phosphoprotein B-50, Neuromodulin, PP46

Gene ID: 2596

NCBI Accession: NP_002036

UniProt: P17677