Cytokeratin 18 antibody (AA 31-35)

Catalog No. ABIN197580

This anti-Cytokeratin 18 antibody is a Rabbit Polyclonal antibody detecting Cytokeratin 18 in WB and IHC (p). Suitable for Human.

Quick Overview for Cytokeratin 18 antibody (AA 31-35) (ABIN197580)

Target: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cytokeratin 18 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Cytokeratin 18 Antibody

Binding Specificity: AA 31-35

Specificity: This antibody detects endogenous levels of total Keratin 18 protein.

Purification: Affinity-Chromatography using epitope-specific immunogen.

Immunogen: Peptide Sequenece around amino acids 31~35 (A-A-S-V-Y) derived from Human Keratin 18.

Application Details

Application Notes: Immunohistochemistry: 1: 50approx. 1: 100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C.

Target Details for Cytokeratin 18

Target: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Alternative Name: Cytokeratin 18

Background: Cytokeratin 18 is an acidic keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas. Cytokeratin 18 exists in combination with Cytokeratin 8, a basic keratin. Hepatocellular carcinomas have been reportedly defined by the use of antibodies that recognize only Cytokeratins 8 and 18.Synonyms: CK18, CYK18, Cell proliferation-inducing gene 46 protein, Cytokeratin-18, K18, KRT18, Keratin 18, Keratin type I cytoskeletal 18, Keratin-18

Gene ID: 3875

NCBI Accession: NP_000215

UniProt: P05783

Pathways: Apoptosis, Caspase Cascade in Apoptosis