KRT8 antibody (Ser73)

Catalog No. ABIN197582

The Rabbit Polyclonal anti-KRT8 antibody has been validated for WB, IF and IHC (p). It is suitable to detect KRT8 in samples from Human.

Quick Overview for KRT8 antibody (Ser73) (ABIN197582)

Target: KRT8 (Keratin 8 (KRT8))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KRT8 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-KRT8 Antibody

Binding Specificity: Ser73

Specificity: This antibody detects endogenous levels of total Keratin 8 protein.

Purification: Affinity chromatography

Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human Keratin 8 around the phosphorylation site of serine 73 (L-L-Sp-P-L).

Application Details

Application Notes: Immunofluorescence: 1/100approx. 1/200. Immunohistochemistry on Paraffin-Embedded Sections: 1/50approx. 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C.

Target Details for KRT8

Target: KRT8 (Keratin 8 (KRT8))

Alternative Name: Cytokeratin 8

Background: Cytokeratin 8 belongs to the type B (basic) subfamily of high molecular weight keratins and exists in combination with cytokeratin 18. Cytokeratin 8 is primarily found in the non squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only cytokeratin polypeptides 8 and 18Synonyms: CK8, CYK8, Cytokeratin endo A, Cytokeratin-8, K8, KRT8, Keratin, Keratin-8, type II cytoskeletal 8

Gene ID: 3856

NCBI Accession: NP_002264

UniProt: P05787