Phosphotyrosine antibody

Catalog No. ABIN197635

This Mouse Monoclonal antibody specifically detects Phosphotyrosine in WB, IF, IP, EIA and IHC (p).

Quick Overview for Phosphotyrosine antibody (ABIN197635)

Target: Phosphotyrosine

Host: Mouse

Clonality: Monoclonal

Conjugate: This Phosphotyrosine antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: PY20

Product Details anti-Phosphotyrosine Antibody

Purification: Affinity Chromatography

Isotype: IgG2b

Application Details

Application Notes: ELISA (0.1 μg/mL)ImmunofluorescenceWestern blotting (2g/mL)Immmunoprecipitation: use Protein A and Ab at 2 g/mg protein lysate. Immunohistochemistry on paraffin embedded sections: 5-10 μg/mL 30 min RT (Staining offormalin-fixed tissues REQUIRES boiling tissue sections in 10 mM citrate buffer, pH 6.0, for10-20 min. followed by cooling at RT for 20 min. )
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Borate buffered saline pH 8.0, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 4 °C for up to one month or aliquot and store at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for Phosphotyrosine

Target: Phosphotyrosine

Target Type: Amino Acid

Background: The phosphorylation of tyrosine acts as a important signal in the control of cell mitogenesis, differentiation, proliferation, and migration and occurs following the activation of intracellular tyrosine kinases, including the T-cell receptor (TCR), epidermal growth factor (EGF) and many families of receptor and non-receptor protein tyrosine kinases (PTKs), which catalyse the transfer of ATP to a tyrosine residue on specific cell protein targets.