BRD4 antibody (AA 1312-1362)

Catalog No. ABIN1977085

This Rabbit Polyclonal antibody specifically detects BRD4 in WB, IHC, IF, IP, IHC (p) and PLA. It exhibits reactivity toward Human, Mouse, Rat, Dog, Cow, Guinea Pig, Rabbit, Horse and Pig.

Quick Overview for BRD4 antibody (AA 1312-1362) (ABIN1977085)

Target: BRD4 (Bromodomain Containing 4 (BRD4))

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Rabbit, Horse, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BRD4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Proximity Ligation Assay (PLA)

Product Details anti-BRD4 Antibody

Binding Specificity: AA 1312-1362

Specificity: Region between residue 1312 and 1362 of human bromodomain-containing protein 4 using the numbering given in entry NP_490597.1 (GeneID 23476).

Purification: Immunoaffinity purified

Isotype: IgG

Application Details

Application Notes: Approved: IF (1:250 - 1:1000), IHC, IHC-P (1:500 - 1:2000), IP (2 - 10 μg/mg lysate), PLA (1:200 - 1:4000), WB (1:10000 - 1:25000)

Usage: Immunohistochemistry: Antigen retrieval with citrate buffer  pH 6.0 is recommended for FFPE tissue sections. Human controls: Breast Carcinoma, Ovarian Carcinoma.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-citrate/phosphate buffer,  pH 7-8, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C for up to 1 year.

Expiry Date: 12 months

Target Details for BRD4

Target: BRD4 (Bromodomain Containing 4 (BRD4))

Alternative Name: BRD4

Background: Name/Gene ID: BRD4
Subfamily: BRD
Family: Protein Kinase

Synonyms: BRD4, Bromodomain containing 4, Chromosome-associated protein, MCAP, HUNK1, Protein HUNK1, Bromodomain-containing 4, HUNKI

Gene ID: 23476

Pathways: Chromatin Binding, SARS-CoV-2 Protein Interactome