p300 antibody (AA 950-1000)

Catalog No. ABIN1978817

The Rabbit Polyclonal anti-p300 antibody has been validated for WB, IHC, IP, IHC (p), IF, ChIP and PLA. It is suitable to detect p300 in samples from Human.

Quick Overview for p300 antibody (AA 950-1000) (ABIN1978817)

Target: p300 (EP300) (E1A Binding Protein P300 (EP300))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This p300 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Proximity Ligation Assay (PLA)

Product Details anti-p300 Antibody

Binding Specificity: AA 950-1000

Specificity: Region between residues 950 and 1000 of human E1Aassociated protein p300 using the numbering given in entry NP_001420.2 (GeneID 2033).

Purification: Immunoaffinity purified

Isotype: IgG

Application Details

Application Notes: Approved: ChrIP, IF (1:500 - 1:2500), IHC, IHC-P (1:1000 - 1:5000), IP (1 - 4 μg/mg lysate), PLA (1:1000 - 1:10000), WB (1:5000 - 1:25000)

Usage: Immunohistochemistry: Antigen retrieval with citrate buffer  pH 6.0 is recommended for FFPE tissue sections. Human controls: Breast Carcinoma, Colon Carcinoma, Lung Adenocarcinoma, Ovarian Carcinoma, Prostate Carcinoma, Small Cell Lung Cancer, Stomach Adenocarcinoma, Testicular Seminoma.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-citrate/phosphate buffer,  pH 7-8, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C for up to 1 year.

Expiry Date: 12 months

Target Details for p300

Target: p300 (EP300) (E1A Binding Protein P300 (EP300))

Alternative Name: EP300 / p300

Background: Name/Gene ID: EP300

Synonyms: EP300, E1A binding protein p300, Histone acetyltransferase p300, KAT3B, p300 HAT, E1A-associated protein p300, E1A-binding protein, 300kD, p300, RSTS2

Gene ID: 2033

Pathways: p53 Signaling, Notch Signaling, Interferon-gamma Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Lipid Metabolism by PPARalpha, Regulation of Muscle Cell Differentiation, Regulation of Cell Size