GATA2 antibody (AA 117-338)

Catalog No. ABIN1980407

The Rabbit Polyclonal anti-GATA2 antibody has been validated for WB, IHC, ICC and IP. It is suitable to detect GATA2 in samples from Mouse. There is 1 publication available.

Quick Overview for GATA2 antibody (AA 117-338) (ABIN1980407)

Target: GATA2 (GATA Binding Protein 2 (GATA2))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GATA2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Product Details anti-GATA2 Antibody

Binding Specificity: AA 117-338

Purpose: Polyclonal Antibody to GATA Binding Protein 2 (GATA2)

Specificity: The antibody is a rabbit polyclonal antibody raised against GATA2. It has been selected for its ability to recognize GATA2 in immunohistochemical staining and western blotting.

Cross-Reactivity: Human, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: GATA2 (Thr117-Arg338)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.01 % SKL, 1 mM DTT, 5 % Trehalose and Proclin300.

Preservative: Dithiothreitol (DTT), ProClin

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-GATA2 antibody (ABIN1980407)

Russo, Mallia, Zito, Lampiasi: "Gene Expression Profiling of NFATc1-Knockdown in RAW 264.7 Cells: An Alternative Pathway for Macrophage Differentiation." in: Cells, Vol. 8, Issue 2, (2019) (PubMed).

Target Details for GATA2

Target: GATA2 (GATA Binding Protein 2 (GATA2))

Alternative Name: GATA2

Background: NFE1B, Endothelial transcription factor GATA-2

Pathways: Stem Cell Maintenance