SPARC antibody (AA 18-273) (APC)

Catalog No. ABIN1980482

The Guinea Pig Polyclonal anti-SPARC antibody is suitable to detect SPARC in samples from Rabbit. It has been validated for WB, IHC, ICC and IP.

Quick Overview for SPARC antibody (AA 18-273) (APC) (ABIN1980482)

Target: SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

Reactivity: Rabbit

Host: Guinea Pig

Clonality: Polyclonal

Conjugate: This SPARC antibody is conjugated to APC

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Product Details anti-SPARC Antibody (APC)

Binding Specificity: AA 18-273

Purpose: Polyclonal Antibody to Osteonectin (ON)

Sequence: MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- APQ QEALPDETEV VEETVAEVAE VAEVPVGANP VQVEVGEFEE VEETEEEVVA ENPCQNHHCK HGKVCELDEN NTPMCVCQDP TSCPAPVGEF EKVCSNDNKT FDSSCHFFAT KCTLEGTKKG HKLHLDYIGP CKYIPPCLDS ELSEFPLRMR DWLKNVLVTL YERDEGNNLL TEKQKLRVKK IHENEKRLEA GDHPVELLAR DFEKNYNMYI FPVHWQFGQL DQHPIDGYLS HTELAPLRAP LIPMEHCTTR FFE

Specificity: The antibody is a cavia polyclonal antibody raised against ON. It has been selected for its ability to recognize ON in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: RPA791Rb01Recombinant Osteonectin (ON)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for SPARC

Target: SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

Alternative Name: Osteonectin

Background: SPARC, BM-40, Secreted Protein,Acidic,Cysteine-Rich, Basement-membrane protein 40, Secreted protein acidic and rich in cysteine

Pathways: Autophagy