PDGFRA antibody (Extracellular Domain)

Catalog No. ABIN198260

The Mouse Monoclonal anti-PDGFRA antibody has been validated for WB, ELISA, IHC and IHC (p). It is suitable to detect PDGFRA in samples from Human.

Quick Overview for PDGFRA antibody (Extracellular Domain) (ABIN198260)

Target: PDGFRA (Platelet Derived Growth Factor Receptor alpha (PDGFRA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PDGFRA antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 35248 (57-20)

Product Details anti-PDGFRA Antibody

Binding Specificity: Extracellular Domain

Specificity: Recognizes the human platelet-derived growth factor receptor alpha, a 1089 amino acid, ~180 kD single pass type I transmembrane glycoprotein. PDGF is a major serum mitogen that can exist as a homo- or heterodimeric protein consisting of disulphide-linked PDGF-A and PDGF-B chains. The PDGF-AA, PDGF-BB and PDGF-AB isoforms have been shown to bind to two distinct cell surface PDGF receptors with different affinities. Whereas PDGF receptor alpha binds all three isoforms with high affinity, PDGF R beta binds PDGF-BB and AB but not PDGF-AA. Both PDGF receptor alpha and beta are members of the class III subfamily of receptor tyrosine kinases. Has been reported for use in functional studies. The ND50 was determined as 0.2-0.6 μg/mL for blocking 10 ng/mL of PDGF-AA biological activity on a quiescent confluent cell culture of human WS1 cells.

Purification: Protein G purified

Immunogen: Purified recombinant human platelet-derived growth factor (PDGF) receptor alpha (extracellular domain), derived from the insect cell line Sf21.

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA (0.5 - 1 μg/mL), IHC, IHC-P (10 - 20 μg/mL), WB (1 - 2 μg/mL)

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.09 % sodium azide, 1.5 % Trehalose

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles. Store undiluted.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Store undiluted.

Target Details for PDGFRA

Target: PDGFRA (Platelet Derived Growth Factor Receptor alpha (PDGFRA))

Alternative Name: PDGFRA / PDGFR alpha

Background: Name/Gene ID: PDGFRA
Subfamily: PDGF Receptor
Family: Protein Kinase

Synonyms: PDGFRA, CD140A, CD140a antigen, PDGFalphaR, PDGFR2, PDGFRalpha, AlphaPDGFR, RHEPDGFRA, PDGF receptor alpha, PDGFR-2, PDGFRA/BCR fusion, APDGFR, Pdgf alpha-receptor, PDGF-R-alpha, PDGFR-alpha

Gene ID: 5156

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Platelet-derived growth Factor Receptor Signaling