IL4 Receptor antibody (Extracellular Domain)

Catalog No. ABIN198262

This Mouse Monoclonal antibody specifically detects IL4 Receptor in WB, ELISA, IHC, FACS and IHC (p). It exhibits reactivity toward Human.

Quick Overview for IL4 Receptor antibody (Extracellular Domain) (ABIN198262)

Target: IL4 Receptor (IL4R) (Interleukin 4 Receptor (IL4R))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL4 Receptor antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 49-20

Product Details anti-IL4 Receptor Antibody

Binding Specificity: Extracellular Domain

Specificity: Recognizes human Interleukin-4 receptor subunit alpha, also known as CD124. CD124 is a 825 amino acid ~90kD type I single pass transmembrane glycoprotein bearing a single domain. Interleukin-4 Receptor form a heterodimeric complex with the, referred to as the IL-4 type-1 complex. The alpha subunit can also form the type-II complex through association with the IL-13 receptor alpha 1 subunit. The IL-4R complex acts as a receptor for both interleukin-4 and interleukin-13. IL-4 R is expressed by B cells, T cells, monocytes and macrophages. Is reported to block human cell surface IL-4 receptor mediated bioactivities induced by IL-4 or IL-13. An ND50 was observed at approximately 5-20 ng/mL in the presence of 0.2 ng/mL of IL-4 using the factor dependant human erythroleukemic cell line, TF-1.

Purification: Protein A purified

Immunogen: Sf 21-derived, recombinant human interleukin 4 receptor, extracellular domain.

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA (1:500 - 1:2000), Flo (1:20 - 1:80), IHC, IHC-P (1:40 - 1:125), WB (1:250 - 1:1000)

Usage: Western Blot: Will detect human IL-4 R under non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.09 % sodium azide, 10 % trehalose

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for IL4 Receptor

Target: IL4 Receptor (IL4R) (Interleukin 4 Receptor (IL4R))

Alternative Name: IL4R / CD124

Background: Name/Gene ID: IL4R
Family: Interleukin

Synonyms: IL4R, CD124, CD124 antigen, IL-4RA, IL4R nirs variant 1, IL4RA, Interleukin 4 receptor, IL-4 receptor subunit alpha, IL-4R subunit alpha, IL-4R-alpha

Gene ID: 3566

Pathways: JAK-STAT Signaling, Positive Regulation of Immune Effector Process