ITGA3 antibody

Catalog No. ABIN198775

This Mouse Monoclonal antibody specifically detects ITGA3 in FACS, IHC, IP, IHC (fro) and Func. It exhibits reactivity toward Human.

Quick Overview for ITGA3 antibody (ABIN198775)

Target: ITGA3 (Integrin, alpha 3 (ITGA3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ITGA3 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)

Product Details anti-ITGA3 Antibody

Specificity: Recognized human a3 integrin (CD49c).

Purification: Protein A purified

Immunogen: Human squamous cell carcinoma cells (SCC9).

Type of Immunogen: Cells

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: Flo, Func, IHC, IHC-Fr, IP

Usage: Suitable for use in Inhibition of Cell Attachment, Flow Cytometry, Immunoprecipitation and Immunohistochemistry. Inhibition of Cell Attachment: Inhibits SCC9 cell adhesion to human and EHS laminin, and laminin GD-2 peptide. Potentiates inhibition of SKOV-3 cell adhesion to EHS laminin by anti-integrin alpha 5 antibody GoH3. Does not inhibit cell adhesion to fibronectin or collagen type IV. Flow Cytometry: Stains human squamous cell carcinoma (SCC9), ovarian carcinoma (SKOV-3) and umbilical vein endothelial cells (HUVEC). Immunohistochemistry: On acetone-fixed frozen human epithelial tissues including tongue, foreskin and ovary. Not for use on formalin-fixed tissue. Immunoprecipitation: Precipitates PAGE bands of 130kD (reduced) or 116kD and 135kD (non-reduced) from surface-biotinylated SCC9 or SKOV-3 cells.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.6, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Aliquot to Avoid repeated freezing and thawing

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C, Long term: aliquot to Avoid freeze-thaw cycles, and store at -20°C.

Target Details for ITGA3

Target: ITGA3 (Integrin, alpha 3 (ITGA3))

Alternative Name: ITGA3 / CD49c

Background: Name/Gene ID: ITGA3
Family: Integrin

Synonyms: ITGA3, Alpha3 integrin, CD49c antigen, CD49C, GAPB3, Integrin alpha-3, FRP-2, VCA-2, VLA-3 subunit alpha, VL3A, VLA3a, Galactoprotein B3, GAP-B3, ILNEB, Integrin alpha3, MSK18

Gene ID: 3675

UniProt: P26006

Pathways: CXCR4-mediated Signaling Events, Integrin Complex