HSPG2 antibody (Domain 5)

Catalog No. ABIN199269

This anti-HSPG2 antibody is a Mouse Monoclonal antibody detecting HSPG2 in WB, IHC, ELISA, IP and IHC (fro). Suitable for Human and Cow.

Quick Overview for HSPG2 antibody (Domain 5) (ABIN199269)

Target: HSPG2 (Heparan Sulfate Proteoglycan 2 (HSPG2))

Reactivity: Human, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This HSPG2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-HSPG2 Antibody

Binding Specificity: Domain 5

Specificity: Highly specific for bovine perlecan. There is no evidence for cross-reactivity with other connective tissue proteins (vitronectin, fibronectin, elastin, collagen, laminin). Species cross-reactivity: human.

Purification: Protein A/G purified

Immunogen: Bovine corneal endothelial cells. Epitope: domain V.

Type of Immunogen: Cells

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA (1:2000), IHC, IHC-Fr, IP, WB (1:50)

Usage: Suitable for use in ELISA, Western Blot, Immunohistochemistry, and Immunoprecipitation. Can also be used in affinity chromatography, in conjunction with clone 6F165 to separate recombinant domain I from full length perlecan. ELISA: 1:2000. Western Blot: 1:50. Immunohistochemistry: 1:50. Frozen PLP-fixed sections of bovine and human tissues.

Comment:

Target Species of Antibody: Bovine

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for HSPG2

Target: HSPG2 (Heparan Sulfate Proteoglycan 2 (HSPG2))

Alternative Name: HSPG2 / Perlecan

Background: Name/Gene ID: HSPG2

Synonyms: HSPG2, Endorepellin (domain V region), HSPG, Heparan sulfate proteoglycan 2, Perlecan, Perlecan proteoglycan, SJA, SJS1, PRCAN, SJS

Gene ID: 3339

UniProt: P98160

Pathways: Glycosaminoglycan Metabolic Process, Lipid Metabolism