HSPG2 antibody (Domain 5)

Catalog No. ABIN199269

The Mouse Monoclonal anti-HSPG2 antibody (ABIN199269) specifically detects HSPG2 in WB, IHC, ELISA, IP and IHC (fro). The antibody is reactive with Human and Cow samples.

Quick Overview for HSPG2 antibody (Domain 5) (ABIN199269)

Target: HSPG2 (Heparan Sulfate Proteoglycan 2 (HSPG2))

Reactivity: Human, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This HSPG2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-HSPG2 Antibody

Binding Specificity: Domain 5

Specificity: Highly specific for bovine perlecan. There is no evidence for cross-reactivity with other connective tissue proteins (vitronectin, fibronectin, elastin, collagen, laminin). Species cross-reactivity: human.

Purification: Protein A/G purified

Immunogen: Bovine corneal endothelial cells. Epitope: domain V.

Type of Immunogen: Cells

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA (1:2000), IHC, IHC-Fr, IP, WB (1:50)

Usage: Suitable for use in ELISA, Western Blot, Immunohistochemistry, and Immunoprecipitation. Can also be used in affinity chromatography, in conjunction with clone 6F165 to separate recombinant domain I from full length perlecan. ELISA: 1:2000. Western Blot: 1:50. Immunohistochemistry: 1:50. Frozen PLP-fixed sections of bovine and human tissues.

Comment:

Target Species of Antibody: Bovine

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for HSPG2

Target: HSPG2 (Heparan Sulfate Proteoglycan 2 (HSPG2))

Alternative Name: HSPG2 / Perlecan

Background: Name/Gene ID: HSPG2

Synonyms: HSPG2, Endorepellin (domain V region), HSPG, Heparan sulfate proteoglycan 2, Perlecan, Perlecan proteoglycan, SJA, SJS1, PRCAN, SJS

Gene ID: 3339

UniProt: P98160

Pathways: Glycosaminoglycan Metabolic Process, Lipid Metabolism