HSPG2 antibody (Domain 3)

Catalog No. ABIN199273

This Mouse Monoclonal antibody specifically detects HSPG2 in WB, IHC, ELISA, IHC (p) and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for HSPG2 antibody (Domain 3) (ABIN199273)

Target: HSPG2 (Heparan Sulfate Proteoglycan 2 (HSPG2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HSPG2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-HSPG2 Antibody

Binding Specificity: Domain 3

Specificity: Recognizes domain III of human perlecan. Domain III shares homology with the short arm of laminin A and B chains.

Purification: Protein A purified

Immunogen: Recombinant Perlecan domain III.

Type of Immunogen: Recombinant protein

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA, IHC, IHC-Fr, IHC-P, WB (1 - 2 μg/mL)

Usage: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Western Blot: 1-2 μg/mL. Immunohistochemistry (Frozen or FFPE): 5-10 μg/mL. Note: Formalin-fixed, paraffin sections require pretreatment with 0.1 % protease for 30 minutes at RT prior to the blocking step. Positive control: Human breast, colon, liver, lymph nodes, pancreas and prostate tissues.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for HSPG2

Target: HSPG2 (Heparan Sulfate Proteoglycan 2 (HSPG2))

Alternative Name: HSPG2 / Perlecan

Background: Name/Gene ID: HSPG2

Synonyms: HSPG2, Endorepellin (domain V region), HSPG, Heparan sulfate proteoglycan 2, Perlecan, Perlecan proteoglycan, SJA, SJS1, PRCAN, SJS

Gene ID: 3339

UniProt: P98160

Pathways: Glycosaminoglycan Metabolic Process, Lipid Metabolism