CD51/CD61 antibody

Catalog No. ABIN199326

This anti-CD51/CD61 antibody is a Mouse Monoclonal antibody detecting CD51/CD61 in FACS, IP, IHC (fro), IF, IHC and ICC. Suitable for Human, Chicken, Rabbit, Cow, Dog, Monkey, Pig and Avian.

Quick Overview for CD51/CD61 antibody (ABIN199326)

Target: CD51/CD61 (ITGAV/ITGB3) (Integrin alpha V, Integrin beta 3 (ITGAV/ITGB3))

Reactivity: Human, Chicken, Rabbit, Cow, Dog, Monkey, Pig, Avian

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD51/CD61 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Clone: LM609

Product Details anti-CD51/CD61 Antibody

Specificity: Recognizes the human vitronectin receptor aVb3 complex, an RGD-directed adhesion receptor. Species cross-reactivity: bovine, chicken, monkey, porcine, canine, rabbit and avian. Not reactive with rat tissue.

Purification: Protein A purified

Immunogen: Purified adhesion receptor from the human melanoma cell line M21.

Type of Immunogen: Cells

Isotype: IgG1

Application Details

Application Notes: Approved: Flo, ICC, IF (5 - 10 μg/mL), IHC, IHC-Fr, IP

Usage: Suitable for use in Immunoprecipitation, Immunofluorescence, Flow Cytometry, Immunohistochemistry (frozen sections only), Immunocytochemistry and Blocking. Immunoprecipitation: Precipitates integrin alpha V beta3 complex. When resolved by SDS-PAGE under reducing conditions, bands are observed at ~130kD corresponding to the alpha V subunit and at ~105kD corresponding to the beta3 subunit. Flow Cytometry: 1-3 μg/0.5x1E6cells in 100 μL volume. Immunofluorescence: 5-10 μg/mL for staining of bFGF-treated 6mm cryosections of chick chorioallantoic membrane fixed with acetone. Also effective on 4 % paraformaldehyde fixed, frozen tissues and cells. Not effective for immunohistochemical staining of paraffin-embedded tissue sections. Blocking: Inhibits adhesion of cells to vitronectin coated surfaces at 10-25 μg/mL. Positive control: Human myeloma cell lines M21 and MoalphaV.

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.1. No preservatives added.

Preservative: Without preservative

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for CD51/CD61

Target: CD51/CD61 (ITGAV/ITGB3) (Integrin alpha V, Integrin beta 3 (ITGAV/ITGB3))

Alternative Name: Integrin Alpha V Beta 3