TYR antibody (AA 1-433)

Catalog No. ABIN199780

This Mouse Monoclonal antibody specifically detects TYR in WB, IHC, IF, IHC (p) and ICC. It exhibits reactivity toward Human, Dog and Cat.

Quick Overview for TYR antibody (AA 1-433) (ABIN199780)

Target: TYR (Tyrosinase (TYR))

Reactivity: Human, Dog, Cat

Host: Mouse

Clonality: Monoclonal

Conjugate: This TYR antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Product Details anti-TYR Antibody

Binding Specificity: AA 1-433

Specificity: Recognizes a cluster of proteins between 70-80kD, identified as tyrosinase. Occasionally a minor band at 55kD is also detected. Does not cross-react with MAGE-1 and tyrosinase-related protein 1, TRP-1/gp75. Species cross-reactivity: Human, canine and feline. Weak cross-reactivity with mouse.

Purification: Protein A purified

Immunogen: Recombinant tyrosinase protein. Epitope: 1-433. Cellular Localization: Cytoplasmic.

Type of Immunogen: Recombinant protein

Isotype: IgG2a

Application Details

Application Notes: Approved: ICC, IF, IHC, IHC-P, WB (1 - 2 μg/mL)

Usage: Suitable for use in Immunocytology, Immunofluorescence, Western Blot and Immunohistology. Western Blot: 1-2 μg/mL for 2 hours at RT. Immunohistology: 2-4 μg/mL for 30 minutes at RT (formalin/paraffin). Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA,  pH 8.0, for 10-20 min followed by cooling at RT for 20 min. Positive control: Melanoma cell lines (SK-MEL-13, SK-MEL-19, SK-MEL-30, SK-MEL-37), melanocytes in skin and Melanomas (1). The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4. No preservatives or stabilizing proteins added.

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for TYR

Target: TYR (Tyrosinase (TYR))

Alternative Name: Tyrosinase

Background: Name/Gene ID: TYR

Synonyms: TYR, CMM8, LB24-AB, SHEP3, SK29-AB, Monophenol monooxygenase, Tumor rejection antigen AB, OCA1A, Tyrosinase, OCAIA

Gene ID: 7299

UniProt: P14679