BUB1B antibody (AA 1-350)

Catalog No. ABIN199935

The Mouse Monoclonal anti-BUB1B antibody (ABIN199935) specifically detects BUB1B in WB, IF and IP. The antibody is reactive with Human samples.

Quick Overview for BUB1B antibody (AA 1-350) (ABIN199935)

Target: BUB1B (Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This BUB1B antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

Product Details anti-BUB1B Antibody

Binding Specificity: AA 1-350

Specificity: Amino acid residues 1-350 of human BUBR1 Kinase protein.

Purification: Protein A purified

Immunogen: Recombinant human BUB1B / BubR1.

Type of Immunogen: Recombinant protein

Application Details

Application Notes: Approved: IF, IP, WB

Usage: This protein A purified antibody has been tested for use in immunoprecipitation, immunofluorescence staining and western blot and is capable of detecting endogenous protein. Specific conditions for reactivity should be optimized by the end user. Expect a predominant band at ~120 kD corresponding to full-length protein by western blotting in the appropriate cell lysate or extract. Higher MW bands may be seen that may be due to hyper-phosphorylation of the protein. The use of HeLa whole cell lysates is recommended as a positive control. For IF microscopy use cells grown on cover slips fixed with 3.5 % paraformaldehyde in PBS at  pH 6.8. Permeabilize fixed cells with 0.2 % Triton X-100 in 25 mM Tris Cl,  pH 7.4 containing 0.1 % BSA.

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for BUB1B

Target: BUB1B (Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B))

Alternative Name: BUB1B / BubR1

Background: Name/Gene ID: BUB1B
Subfamily: BUB1
Family: Protein Kinase

Synonyms: BUB1B, Bub1A, BUBR1, HBUBR1, Protein SSK1, SSK1, BUB1beta, MAD3L

Gene ID: 701

UniProt: O60566