N-Cadherin antibody (Intracellular)

Catalog No. ABIN200337

This anti-N-Cadherin antibody is a Mouse Monoclonal antibody detecting N-Cadherin in WB, IHC, IF, IP and IHC (p). Suitable for Human, Mouse, Rat, Chicken and Pig.

Quick Overview for N-Cadherin antibody (Intracellular) (ABIN200337)

Target: N-Cadherin (CDH2) (Cadherin 2 (CDH2))

Reactivity: Human, Mouse, Rat, Chicken, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This N-Cadherin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-N-Cadherin Antibody

Binding Specificity: Intracellular

Specificity: Recognizes chicken N-cadherin. Does not cross-react with other cadherin family members including P- and E-cadherins. Species cross-reactivity by Western Blot: human mouse, rat and porcine, by Immunofluorescence, Immunohistochemistry (paraffin) and Immunoprecipitation: human.

Purification: Protein A purified

Immunogen: Recombinant protein representing a portion of the intracellular domain of chicken N-cadherin.

Type of Immunogen: Recombinant protein

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: IF (1 - 3 μg/mL), IHC, IHC-P, IP, WB (0.5 - 1 μg/mL)

Usage: Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation and Immunohistochemistry. Immunofluorescence: 1-3 μg/mL. Western Blot: 0.5-1 μg/mL. Immunoprecipitation: 3-5 μg/IP reaction. Immunohistochemistry: 1-5 μg/mL (Formalin fixed, paraffin embedded tissue, Heat induced epitope retrieval required).

Comment:

Target Species of Antibody: Chicken

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for N-Cadherin

Target: N-Cadherin (CDH2) (Cadherin 2 (CDH2))

Alternative Name: CDH2 / N Cadherin

Background: Name/Gene ID: CDH2
Family: Cadherin

Synonyms: CDH2, CDw325, CDHN, Cadherin-2, CD325, CD325 antigen, N Cadherin, N-cadherin, NCAD, Neural cadherin, N-cadherin 1, Neural-cadherin

Gene ID: 1000

UniProt: P19022

Pathways: Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Synaptic Membrane