NG2 antibody (N-Term)

Catalog No. ABIN200588

The Mouse Monoclonal anti-NG2 antibody has been validated for WB, IP, ELISA and IF. It is suitable to detect NG2 in samples from Rat.

Quick Overview for NG2 antibody (N-Term) (ABIN200588)

Target: NG2 (MCSP) (Melanoma Associated Chondroitin Sulfate Proteoglycan (MCSP))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This NG2 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), ELISA, Immunofluorescence (IF)

Product Details anti-NG2 Antibody

Binding Specificity: N-Term

Specificity: This antibody consists of a cocktail of four clones, and will react with the N-terminal domain of NG2, the central collagen binding domain and a membrane proximal epitope. On Western blots, it identifies a band at ~300kD. Reactivity has been confirmed with rat B49 glial cell line.

Purification: Protein A purified

Immunogen: Purified rat NG2 protein.

Type of Immunogen: Purified protein

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA (0.1 - 1 μg/mL), IF (1 - 5 μg/mL), IP, WB (1 - 3 μg/mL)

Usage: Suitable for use in Immunoprecipitation, Immunofluorescence, ELISA and Western Blot. Immunoprecipitation: 10 μg/IP reaction. Immunofluorescence: 1-5 μg/mL. ELISA: 0.1-1 μg/mL. Western Blot: 1-3 μg/mL.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for NG2

Target: NG2 (MCSP) (Melanoma Associated Chondroitin Sulfate Proteoglycan (MCSP))

Alternative Name: CSPG4 / NG2

Background: Name/Gene ID: CSPG4

Synonyms: CSPG4, MCSP, MEL-CSPG, MSK16, NG2, HMW-MAA, MCSPG

Gene ID: 1464

UniProt: Q6UVK1

Pathways: Glycosaminoglycan Metabolic Process