GJA3 antibody (Internal Region)

Catalog No. ABIN200660

The Rabbit Polyclonal anti-GJA3 antibody has been validated for WB, IHC, IF and IHC (fro). It is suitable to detect GJA3 in samples from Mouse.

Quick Overview for GJA3 antibody (Internal Region) (ABIN200660)

Target: GJA3 (Gap Junction Protein, alpha 3, 46kDa (GJA3))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GJA3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-GJA3 Antibody

Binding Specificity: Internal Region

Specificity: Recognizes mouse Connexin 46 protein. Species sequence homology: rat, Chinese hamster and Golden Hamster.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide derived from an internal region of the mouse Connexin 46 protein.

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IF (3 μg/mL), IHC, IHC-Fr, WB (1 - 3 μg/mL)

Usage: Suitable for use in Immunohistochemistry, Western Blot and Immunofluorescence. Immunohistochemistry (Frozen): 3 μg/mL. Western Blot: 1-3 μg/mL. Identifies a single band at ~46kD. Immunofluorescence: 3 μg/mL. Note: For IHC, mice were perfused transcardially with 3 mL pre-fixative (50 mM phosphate buffer, 0.1 % sodium nitrite, 1 unit/mL heparin), followed by 40 mL fixative (0.16M sodium phosphate buffer,  pH 7.6, 0.2 % picric acid, and 1-, 2-, or 4 % paraformaldehyde), and perfusion with 10 mL sucrose wash (10 % sucrose in 25 mM sodium phosphate buffer,  pH 7.4. Tissues were then stored in cryoprotectant (10 % sucrose, 25 mM phosphate buffer,  pH 7.4, 0.04 % sodium azide) for a minimum of 24 hours prior to sectioning.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for GJA3

Target: GJA3 (Gap Junction Protein, alpha 3, 46kDa (GJA3))

Alternative Name: GJA3 / CX46 / Connexin 46

Background: Name/Gene ID: GJA3
Subfamily: Connexin
Family: Ion Channel

Synonyms: GJA3, Connexin-46, CX46, CZP3, Gap junction alpha-3 protein, Gap junction protein alpha 3, CAE3, Connexin 46

Gene ID: 2700

UniProt: Q9Y6H8