GJA3 antibody (Internal Region)

Catalog No. ABIN200660

Product Details anti-GJA3 Antibody

Target: GJA3 (Gap Junction Protein, alpha 3, 46kDa (GJA3))

Binding Specificity: Internal Region

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GJA3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: Recognizes mouse Connexin 46 protein. Species sequence homology: rat, Chinese hamster and Golden Hamster.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide derived from an internal region of the mouse Connexin 46 protein.

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IF (3 μg/mL), IHC, IHC-Fr, WB (1 - 3 μg/mL)

Usage: Suitable for use in Immunohistochemistry, Western Blot and Immunofluorescence. Immunohistochemistry (Frozen): 3 μg/mL. Western Blot: 1-3 μg/mL. Identifies a single band at ~46kD. Immunofluorescence: 3 μg/mL. Note: For IHC, mice were perfused transcardially with 3 mL pre-fixative (50 mM phosphate buffer, 0.1 % sodium nitrite, 1 unit/mL heparin), followed by 40 mL fixative (0.16M sodium phosphate buffer,  pH 7.6, 0.2 % picric acid, and 1-, 2-, or 4 % paraformaldehyde), and perfusion with 10 mL sucrose wash (10 % sucrose in 25 mM sodium phosphate buffer,  pH 7.4. Tissues were then stored in cryoprotectant (10 % sucrose, 25 mM phosphate buffer,  pH 7.4, 0.04 % sodium azide) for a minimum of 24 hours prior to sectioning.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for GJA3

Target: GJA3 (Gap Junction Protein, alpha 3, 46kDa (GJA3))

Alternative Name: GJA3 / CX46 / Connexin 46 (GJA3 Products)

Synonyms: cx46 antibody, MGC53082 antibody, czp3 antibody, Cx44 antibody, cx48.5 antibody, Cnx46 antibody, Cx43 antibody, Cx46 antibody, Gja-3 antibody, CTRCT14 antibody, CX46 antibody, CZP3 antibody, MGC69466 protein L homeolog antibody, gap junction protein alpha 3 antibody, gap junction protein, alpha 3 antibody, MGC69466.L antibody, gja3 antibody, GJA3 antibody, Gja3 antibody

Background: Name/Gene ID: GJA3
Subfamily: Connexin
Family: Ion Channel

Synonyms: GJA3, Connexin-46, CX46, CZP3, Gap junction alpha-3 protein, Gap junction protein alpha 3, CAE3, Connexin 46

Gene ID: 2700

UniProt: Q9Y6H8