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GJA8 antibody (AA 290-440)

This Mouse Monoclonal antibody specifically detects GJA8 in ELISA, WB, IF and IP. It exhibits reactivity toward Human, Mouse, Rat and Sheep.
Catalog No. ABIN200662

Quick Overview for GJA8 antibody (AA 290-440) (ABIN200662)

Target

See all GJA8 Antibodies
GJA8 (Gap Junction Protein, alpha 8, 50kDa (GJA8))

Reactivity

  • 30
  • 17
  • 5
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Human, Mouse, Rat, Sheep

Host

  • 30
  • 1
  • 1
Mouse

Clonality

  • 31
  • 1
Monoclonal

Conjugate

  • 17
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This GJA8 antibody is un-conjugated

Application

  • 20
  • 16
  • 10
  • 3
  • 1
  • 1
  • 1
ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone

C6
  • Binding Specificity

    • 9
    • 6
    • 5
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 290-440

    Specificity

    Specific for Connexin 50. It does not cross-react with other Connexin proteins. Anti-Connexin 50 recognizes an epitope in the cytoplasmic (carboxy) tail located between residues 290-440. Reacts with Connexin 50 from sheep, human, mouse, rat and probably other species. Tested in a wide variety of tissues including lens, cornea and heart valve.

    Purification

    Gel filtration

    Immunogen

    Urea/alkali stripped sheep lens membranes.

    Type of Immunogen: Membrane extract

    Isotype

    IgM kappa
  • Application Notes

    Approved: ELISA (0.1 - 1 μg/mL), IF (10 - 50 μg/mL), IP, WB (1 - 5 μg/mL)

    Usage: Suitable for use in Immunofluorescence, ELISA, Western Blot, Immunoprecipitation. ELISA: 0.1-1.0 μg/mL. Western Blot: 1-5 μg/mL. Immunofluorescence 10-50 μg/mL. Immunoprecipitation: 5-10 μg.

    Comment

    Target Species of Antibody: Sheep

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    Lot specific

    Buffer

    PBS,  pH 7.2, 0.1 % sodium azide.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    4 °C,-20 °C

    Storage Comment

    Short term: 4°C
    Long term: Add glycerol (40-50%) -20°C.
  • Target

    GJA8 (Gap Junction Protein, alpha 8, 50kDa (GJA8))

    Alternative Name

    GJA8 / CX50 / Connexin 50

    Background

    Name/Gene ID: GJA8
    Subfamily: Connexin
    Family: Ion Channel

    Synonyms: GJA8, Connexin 49, Connexin-50, CAE, CX50, Gap junction alpha-8 protein, Gap junction protein alpha 8, Lens fiber protein MP70, MP70, CAE1, Cell surface glycoprotein, Connexin 50, CZP1

    Gene ID

    2703

    UniProt

    P48165
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