EPH Receptor A3 antibody (C-Term)

Catalog No. ABIN200834

The Mouse Monoclonal anti-EPH Receptor A3 antibody has been validated for WB, ELISA and IP. It is suitable to detect EPH Receptor A3 in samples from Human.

Quick Overview for EPH Receptor A3 antibody (C-Term) (ABIN200834)

Target: EPH Receptor A3 (EPHA3)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This EPH Receptor A3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Product Details anti-EPH Receptor A3 Antibody

Binding Specificity: C-Term

Specificity: On Western Blot analysis, this antibody reacts specifically with recombinant human EphA3 receptor without cross-reaction with EphA4 receptor expressed in transfected HEK-293 cells. The antibody reactivity was confirmed by Western Blot and Immunoprecipitation analyses. Reactivity has been confirmed with human EphA3 receptor transfected HEK-293 cell lysate. The molecular weight for human EphA3 receptor is ~110kD on Western blots. Based on sequence homology, the antibody should also recognize the rat or mouse EphA3 receptor.

Purification: Protein A purified

Immunogen: Synthetic peptide derived from the C-terminal region of the human EphA3 receptor.

Type of Immunogen: Synthetic peptide

Isotype: IgG2a kappa

Application Details

Application Notes: Approved: ELISA (0.1 - 1 μg/mL), IP, WB (1 - 3 μg/mL)

Usage: Suitable for use in ELISA, Western Blot and Immunoprecipitation. ELISA: 0.1-1 μg/mL. Western Blot: 1-3 μg/mL, Band at ~110kD. Immunoprecipitation: 5-10 μg. Positive control: Human EphA3 receptor transfected HEK-293 cell lysate.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for EPH Receptor A3

Target: EPH Receptor A3 (EPHA3)

Alternative Name: EPHA3 / EPH Receptor A3

Background: Name/Gene ID: EPHA3
Subfamily: Ephrin Receptor
Family: Protein Kinase

Synonyms: EPHA3, CEK4 kinase, EPH receptor A3, Ephrin type-A receptor 3, Eph-like tyrosine kinase 1, ETK1, HEK4, EPH-like kinase 4, HEK, Human embryo kinase 1, MEK4 kinase, Human embryo kinase, Tyrosine-protein kinase TYRO4, TYRO4, EK4, Eph kinase A3, ETK

Gene ID: 2042

UniProt: P29320

Pathways: RTK Signaling, Regulation of Cell Size