EPH Receptor B4 antibody (C-Term)

Catalog No. ABIN200838

This anti-EPH Receptor B4 antibody is a Mouse Monoclonal antibody detecting EPH Receptor B4 in WB, ELISA, IHC, IHC (p) and IP. Suitable for Human.

Quick Overview for EPH Receptor B4 antibody (C-Term) (ABIN200838)

Target: EPH Receptor B4 (EPHB4)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This EPH Receptor B4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Clone: 3D7G8

Product Details anti-EPH Receptor B4 Antibody

Binding Specificity: C-Term

Specificity: Recognizes human endogenous EphB4 receptor at ~110-120kD, with weak cross-reactivity with the EphB2 receptor.

Purification: Protein A purified

Immunogen: Recombinant protein derived from the C-terminal region of the human EphB4 protein.

Type of Immunogen: Recombinant protein

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P, IP, WB (1 - 3 μg/mL)

Usage: Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Western Blot: 1-3 μg/mL. Immunoprecipitation: 5 μg/IP reaction. Immunohistochemistry: 10 μg/mL. Formalin-fixed, paraffin-embedded tissues require heat induced epitope retrieval (HIER) with citrate buffer,  pH 6.0 prior to staining. Positive control: Western Blot: EphB4-transfected 293T cells and human MDA-MB-231 breast carcinoma cell lysates.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles. Store undiluted.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Store undiluted.

Target Details for EPH Receptor B4

Target: EPH Receptor B4 (EPHB4)

Alternative Name: EPHB4 / EPH Receptor B4

Background: Name/Gene ID: EPHB4
Subfamily: Ephrin Receptor
Family: Protein Kinase

Synonyms: EPHB4, EPH receptor B4, Ephrin receptor EphB4, Ephrin type-B receptor 4, HTK, MYK1, TYRO11, Soluble EPHB4 variant 1, Soluble EPHB4 variant 3, Hepatoma transmembrane kinase, MDK2, Soluble EPHB4 variant 2, Tyrosine-protein kinase TYRO11

Gene ID: 2050

UniProt: P54760

Pathways: RTK Signaling