Glycogen Synthase 1 antibody (pSer640)

Catalog No. ABIN200991

This anti-Glycogen Synthase 1 antibody is a Rabbit Polyclonal antibody detecting Glycogen Synthase 1 in WB and ELISA. Suitable for Human.

Quick Overview for Glycogen Synthase 1 antibody (pSer640) (ABIN200991)

Target: Glycogen Synthase 1 (GYS1) (Glycogen Synthase 1 (Muscle) (GYS1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Glycogen Synthase 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Glycogen Synthase 1 Antibody

Binding Specificity: AA 631-642, pSer640

Specificity: Specific for the phosphorylated form of human muscle GS at S640. Minimally reactive (less than 1 % ) with the non-phosphorylated form of the immunizing peptide. Species cross-reactivity: Human and mouse.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Elephant (100%) Marmoset, Mouse, Rat, Hamster, Panda, Dog, Bovine, Horse, Rabbit, Pig, Xenopus (90%) Opossum, Zebrafish, Fusarium (80%).

Purification: Immunoaffinity purified

Immunogen: Human Muscle Glycogen Synthase phospho-peptide corresponding to a region of the human protein conjugated to (KLH). Sequence: AQGYRYPRPA-(pS)-VP (aa631-642). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Elephant (100%), Marmoset, Mouse, Rat, Hamster, Panda, Dog, Bovine, Horse, Rabbit, Pig, Xenopus (90%), Opossum, Zebrafish, Fusarium (80%).

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (1:30000 - 1:150000), WB (1:1000)

Usage: Suitable for use in Western Blot and ELISA. Recommended Dilution (s): ELISA: 1:30000-1:150000, Assayed against 0.1 μg of phosphorylated peptide in a standard capture ELISA using TMB (3,3',5,5'-Tetramethylbenizidine) as a substrate for 30 minutes at RT. Western Blot: 1:1000.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.01 % sodium azide. No stabilizing proteins added.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for Glycogen Synthase 1

Target: Glycogen Synthase 1 (GYS1) (Glycogen Synthase 1 (Muscle) (GYS1))

Alternative Name: GYS1 / Glycogen Synthase

Background: Name/Gene ID: GYS1

Synonyms: GYS1, Glycogen synthase 1 (muscle), GSY, GYS

Gene ID: 2997

UniProt: P13807

Pathways: PI3K-Akt Signaling, AMPK Signaling, Cellular Glucan Metabolic Process