Laminin antibody

Catalog No. ABIN201206

The Rabbit Polyclonal anti-Laminin antibody is suitable to detect Laminin in samples from Human and Mammalian. It has been validated for WB, IHC, IP, ELISA and IHC (p).

Quick Overview for Laminin antibody (ABIN201206)

Target: Laminin (LN)

Reactivity: Human, Mammalian

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Laminin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Laminin Antibody

Specificity: Recognizes human laminin. Typically less than 1 % cross-reactivity against other extracellular matrix proteins was detected by ELISA against purified standards. Recognizes most mammalian Laminins and has negligible cross-reactivity with Type I, II, III, IV, V or VI Collagens or Fibronectin. Non-specific cross-reaction of anti-Laminin antibodies with other human serum proteins or non-Laminin extracellular matrix proteins is negligible.

Purification: Affinity purified

Immunogen: Synthetic peptide corresponding to regions of adherence on laminin.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (1:2000 - 1:4000), IHC, IHC-P, IP, WB (1:250 - 1:1000)

Usage: Suitable for use in ELISA, Western Blot, Immunoprecipitation, and Immunohistochemistry. ELISA: 1:2000-1:4000. Western Blot: 1:250-1:1000. Immunohistochemistry (paraffin embedded sections): 1:25-1:100.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 125 mM sodium borate, 75 mM sodium chloride, 5 mM EDTA,  pH 8.0, 0.01 % sodium azide, 40 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Laminin

Target: Laminin (LN)

Alternative Name: Laminin

UniProt: P25391