RPS27 antibody (N-Term)

Catalog No. ABIN201320

The Mouse Monoclonal anti-RPS27 antibody has been validated for WB, ELISA, IF and IP. It is suitable to detect RPS27 in samples from Human.

Quick Overview for RPS27 antibody (N-Term) (ABIN201320)

Target: RPS27 (Ribosomal Protein S27 (RPS27))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This RPS27 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP)

Product Details anti-RPS27 Antibody

Binding Specificity: N-Term

Specificity: This antibody recognizes the N-terminus of the ~90kD human Mps1 kinase. Reactivity was confirmed with nocodazole treated HeLa cell lysates.

Purification: Protein A purified

Immunogen: His6-tagged human Mps1 recombinant protein.

Type of Immunogen: Recombinant protein

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA (0.1 - 1 μg/mL), IF (5 - 10 μg/mL), IP, WB (1 - 3 μg/mL)

Usage: Suitable for use in Immunofluorescence, Immunoprecipitation, Western Blot and ELISA. Immunofluorescence: 5-10 μg/mL. Immunoprecipitation: 5-10 μg/ IP reaction. Western Blot: 1-3 μg/mL. ELISA: 0.1-1 μg/mL. Note: For immunofluorescent assays, it is recommended that cells are fixed in 4 % paraformaldehyde in PBS and permeabilized with 0.2 % Triton X-100. Methanol and acetone fixatives can also be used.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for RPS27

Target: RPS27 (Ribosomal Protein S27 (RPS27))

Alternative Name: RPS27 / MPS1

Background: Name/Gene ID: RPS27

Synonyms: RPS27, 40S ribosomal protein S27, Metallopanstimulin 1, MPS1, Ribosomal protein S27, Metallopan-stimulin 1, MPS-1, S27

Gene ID: 6232

UniProt: P42677