MYOD1 antibody (AA 180-189)

Catalog No. ABIN201329

The Mouse Monoclonal anti-MYOD1 antibody is suitable to detect MYOD1 in samples from Human, Mouse, Rat and Cat. It has been validated for WB, IHC, IP and ICC.

Quick Overview for MYOD1 antibody (AA 180-189) (ABIN201329)

Target: MYOD1 (Myogenic Differentiation 1 (MYOD1))

Reactivity: Human, Mouse, Rat, Cat

Host: Mouse

Clonality: Monoclonal

Conjugate: This MYOD1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-MYOD1 Antibody

Binding Specificity: AA 180-189

Specificity: Recognizes MyoD. Epitope Mapping: aa180-189 of mouse MyoD. Species cross-reactivity: Human (myf3), rat, and feline homologs.

Purification: Protein G purified

Immunogen: Recombinant mouse MyoD protein.

Type of Immunogen: Recombinant protein

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ICC (5 μg/mL), IHC, IP (1 μg/mL), WB (1 μg/mL)

Usage: Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Western Blot: 1 μg/mL. Detects ~45kD band. Immunoprecipitation: 1 μg/mL. Immunohistochemistry, Immunocytochemistry: 5 μg/mL. Positive control: Rhabdomyosarcoma cell line (Rh30) whole cell lysate. Does not detect MyoD in primitive neuroectodermal (PFSK-1A), lymphoma (EB2), neuroblastoma (SK-N-SH) and Ewing's sarcoma (SJSA-1) cell lines.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.05 % BSA, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for MYOD1

Target: MYOD1 (Myogenic Differentiation 1 (MYOD1))

Alternative Name: MYOD / MYOD1

Background: Name/Gene ID: MYOD1

Synonyms: MYOD1, BHLHc1, Myogenic differentiation 1, Myf-3, MYOD, Myogenic factor 3, PUM, MYF3

Gene ID: 4654

UniProt: P15172

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development