Nestin antibody

Catalog No. ABIN201334

This Mouse Monoclonal antibody specifically detects Nestin in WB, IHC, ICC and IHC (fro). It exhibits reactivity toward Mouse and Rat.

Quick Overview for Nestin antibody (ABIN201334)

Target: Nestin (NES)

Reactivity: Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Nestin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Nestin Antibody

Specificity: Recognizes rodent Nestin at 200-220kD. Specificity confirmed with mouse and rat. Does not cross-react with human.

Purification: Purified

Immunogen: Nestin purified from embryonic rat spinal cord.

Type of Immunogen: Purified protein

Isotype: IgG1

Application Details

Application Notes: Approved: ICC (1:20 - 1:200), IHC, IHC-Fr (1:10 - 1:100), WB

Usage: Suitable for use in Immunohistochemistry, Western Blot and Immunocytochemistry. Immunohistochemistry (frozen or paraffin): 1:20-1:200. Perfuse tissue with 4 % paraformaldehyde,  pH 7.4 for light microscopy or with either 4 % paraformaldehyde,  pH 10 or 4 % paraformaldehyde with 0.1 % glutaraldehyde,  pH 7.4 for EM. Immunocytochemistry: 1:20-1:200. Fix cells in 4 % paraformaldehyde buffered with 50 mM sodium borate,  pH 9.5. Western Blot: 200-220kD in reducing gels of newborn rat or mouse cell extracts. Prepare samples by boiling in 4 volumes of 125 mM Tris,  pH 6.8, 10 % 2-mercaptoethanol, 10 % glycerol, 4.6 % SDS. 5 % PAGE gels are suggested. Block membranes with milk or BSA. Do not dilute primary antibody in buffer containing milk. Freezing and thawing of samples or samples not stored at -70?C will show breakdown of nestin sometimes into multiple fragments. Positive control: Mouse embryonic brain tissue.

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-glycine, 0.15 M sodium chloride,  pH 7.4, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Nestin

Target: Nestin (NES)

Alternative Name: Nestin

Background: Name/Gene ID: NES

Synonyms: NES, Nestin, Nbla00170

Gene ID: 10763

UniProt: P48681