Paxillin antibody

Catalog No. ABIN201403

This Mouse Monoclonal antibody specifically detects Paxillin in WB, ELISA, IHC, IP and ICC. It exhibits reactivity toward Human, Mouse, Rat, Chicken and Dog.

Quick Overview for Paxillin antibody (ABIN201403)

Target: Paxillin (PXN)

Reactivity: Human, Mouse, Rat, Chicken, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This Paxillin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-Paxillin Antibody

Specificity: Reacts strongly with two bands on Western blots of total cell lysate from chick embryo fibroblasts or rat brain. The lower molecular band is believed to be either an isoform or a degradation product of the 68kD protein, since it is found also on SDS-PAGE of affinity purified paxillin. Recognizes human, canine, mouse, chick and rat.

Purification: Purified

Immunogen: Affinity purified phosphotyrosine-containing proteins from Rous sarcoma virus transformed chick embryo fibroblasts.

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA, ICC, IHC, IP, WB (1:1000 - 1:10000)

Usage: Western blot: 1:1000-1:10000. ELISA. Immunohistochemistry. Immunocytochemistry. Immunoprecipitation. Control Lysate: A431 Lysate: Prepared from a human epidermoid carcinoma cell line. Cell lysate is available to be used as a positive control in Western blotting. Cell lysate is provided at a concentration of 1 μg/mL in SDS-PAGE buffer (62 mM Tris  pH 6.8, 2 % SDS, 0.9 % beta mercaptoethanol, 0.003 % bromophenol blue and 5 % glycerol). 5-10 μL/lane is sufficient for Western blot.

Comment:

Target Species of Antibody: Chicken

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 50 % glycerol, 20 mM sodium phosphate,  pH 7.5, 150 mM sodium chloride, 1.5 mM sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for Paxillin

Target: Paxillin (PXN)

Alternative Name: PXN / Paxillin

Background: Name/Gene ID: PXN

Synonyms: PXN, Paxillin

Gene ID: 5829

UniProt: P49023

Pathways: MAPK Signaling, EGFR Signaling Pathway, Response to Growth Hormone Stimulus, Cell-Cell Junction Organization, Maintenance of Protein Location, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling