Topoisomerase II alpha antibody (C-Term)

Catalog No. ABIN201464

The Mouse Monoclonal anti-Topoisomerase II alpha antibody has been validated for WB, IHC, FACS, IHC (p), IP and ICC. It is suitable to detect Topoisomerase II alpha in samples from Human.

Quick Overview for Topoisomerase II alpha antibody (C-Term) (ABIN201464)

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Topoisomerase II alpha antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-Topoisomerase II alpha Antibody

Binding Specificity: C-Term

Specificity: Recognizes a major protein of 170kD which was identified as the human alpha isoform of topoisomerase II. Recognizes a carboxy terminal alpha-isoenzyme specific epitope missing in topoisomerase II beta.

Purification: Purified

Immunogen: Nuclear protein preparation from the human cell line U937.50.

Type of Immunogen: Cells

Isotype: IgG2a

Application Details

Application Notes: Approved: Flo, ICC (5 - 10 μg/mL), IHC, IHC-P, IP, WB (1 - 10 μg/mL)

Usage: Suitable for use in Immunohistochemistry, Western Blot, Immunoprecipitation, Immunocytochemistry and Flow Cytometry. Western Blot: 1-10 μg/mL. Immunohistochemistry: 5-10 μg/mL. Strong nuclear staining only in proliferating cells. The epitope recognized by the antibody is also detectable in paraffin-embedded tissue sections. Immunocytochemistry: 5-10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.6, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Topoisomerase II alpha

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Alternative Name: TOP2A / Topoisomerase II alpha

Background: Name/Gene ID: TOP2A
Family: Topoisomerase

Synonyms: TOP2A, DNA topoisomerase II, 170 kD, TOP2, DNA gyrase, DNA topoisomerase 2-alpha, TP2A

Gene ID: 7153

UniProt: P11388

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases