Complement C4d antibody

Catalog No. ABIN201862

This Mouse Monoclonal antibody specifically detects Complement C4d in IF, ELISA, IHC, WB, IHC (p) and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for Complement C4d antibody (ABIN201862)

Target: Complement C4d (C4d) (Complement Component C4d (C4d))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Complement C4d antibody is un-conjugated

Application: Immunofluorescence (IF), ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 10-11

Product Details anti-Complement C4d Antibody

Specificity: Recognizes the secreted protein complement component 4d (C4d). The presence of C4d in renal peritubular capillaries is a key indicator for acute antibody-mediated rejection [AMR]. C4d was accepted in 2003 into the Banff classification for identification of acute AMR. Is specific for C4d, a marker that can be used in the detection of acute AMR for kidney, heart, pancreas and lung allografts. C4d is regarded as a key marker of antibody-mediated cell injury and humoral rejection. Complement 1 complex cleaves complement 4 (C4) to form C4b and C4a. C4b levels are strictly regulated. Single site cleavage of the C4ba€™s alpha chain by Factor I forms iC4b and blocks C3 convertase, inhibiting opsonization and activation of the classical pathway. This requires C4 binding protein or CR1 as a cofactor. iC4b is further degraded into C4d and C4c. C4ba€™s short half life means that C4d is present in serum at high enough concentrations to make it a useful marker for classical complement activation. Is used to detect the biomarker C4d which has been described as a a€oefootprinta€ of antibody mediated tissue rejection. The internal thioester of C4b becomes exposed during cleavage to C4d and forms a covalent bond with the cell surface. The longer half-life of covalently bound C4d makes it a footprint of complement activation long after weakly bound antibodies have been cleared by the blood stream. C4 has also been linked to susceptibility to systemic lupus erythematosus and rheumatoid arthritis.

No Cross-Reactivity: Mouse (Murine), Cow (Bovine), Guinea Pig, Cat (Feline), Rabbit, Sheep (Ovine), Dog (Canine), Rat (Rattus)

Purification: Protein A purified

Immunogen: Native, from human plasma.

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA (1:5000 - 1:20000), IF (1:250 - 1:600), IHC, IHC-Fr (1:100 - 1:750), IHC-P, WB

Usage: Immunohistochemistry: It has been reported that this antibody works very well on acetone-fixed, frozen renal biopsies. Strong staining is observed in the glomeruli and in some cases the peritubular capillaries. Clone 10-11 has given variable results on formalin-fixed, paraffin-embedded sections. It has been observed that pre-treatment with 88 % formic acid for 20 minutes at room temperature is beneficial (6).

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Borate buffered saline,  pH 8.4, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Short term: -20°C
Long term: -20°C.

Target Details for Complement C4d

Target: Complement C4d (C4d) (Complement Component C4d (C4d))

Alternative Name: Complement C4d