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SMAD3 antibody (N-Term)

The Rabbit Polyclonal anti-SMAD3 antibody has been validated for WB, IHC and IHC (p). It is suitable to detect SMAD3 in samples from Human, Mouse, Rat, Pig, Chicken, Monkey, Zebrafish (Danio rerio), Dog, Xenopus laevis and Guinea Pig.
Catalog No. ABIN202493

Quick Overview for SMAD3 antibody (N-Term) (ABIN202493)

Target

See all SMAD3 Antibodies
SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Reactivity

  • 229
  • 156
  • 138
  • 26
  • 8
  • 8
  • 7
  • 7
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  • 4
  • 3
  • 2
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  • 1
  • 1
  • 1
Human, Mouse, Rat, Pig, Chicken, Monkey, Zebrafish (Danio rerio), Dog, Xenopus laevis, Guinea Pig

Host

  • 217
  • 29
  • 1
Rabbit

Clonality

  • 200
  • 47
Polyclonal

Conjugate

  • 128
  • 14
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  • 4
This SMAD3 antibody is un-conjugated

Application

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Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • Binding Specificity

    • 40
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    • 27
    • 27
    • 25
    • 12
    • 11
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    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
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    • 1
    • 1
    • 1
    • 1
    • 1
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    • 1
    • 1
    • 1
    • 1
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    • 1
    • 1
    N-Term

    Specificity

    Human SMAD3

    Predicted Reactivity

    Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Pig, Guinea pig, Chicken, Xenopus, Zebrafish (100%).

    Purification

    Protein A purified

    Immunogen

    Synthetic peptide from N-Terminus of human SMAD3 (NP_005893). Percent identity by BLAST analysis: Human, Chimpanzee, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Pig, Guinea pig, Zebra finch, Chicken, Xenopus, Trout, Zebrafish (100%).

    Type of Immunogen: Synthetic peptide

    Isotype

    IgG
  • Application Notes

    Approved: IHC, IHC-P, WB (2 μg/mL)

    Comment

    Target Species of Antibody: Human

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    After adding water, will consist of PBS buffer with 2 % sucrose

    Concentration

    Lot specific

    Buffer

    Lyophilized.

    Handling Advice

    Avoid repeat freeze-thaw cycles.

    Storage

    4 °C,-20 °C

    Storage Comment

    Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
    Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
  • Target

    SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

    Alternative Name

    SMAD3

    Background

    Name/Gene ID: SMAD3

    Synonyms: SMAD3, HSPC193, HMAD-3, HsT17436, LDS1C, LDS3, Mad homolog JV15-2, MAD3, MADH3, Mothers against DPP homolog 3, JV15-2, SMA- and MAD-related protein 3, SMAD family member 3, MAD homolog 3, HSMAD3, Mad protein homolog, SMAD 3

    Gene ID

    4088

    NCBI Accession

    NP_005893

    UniProt

    P84022

    Pathways

    Cell Division Cycle, Chromatin Binding, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Autophagy
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